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slow twitch myosin heavy chain myhc type i  (Developmental Studies Hybridoma Bank)


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    Developmental Studies Hybridoma Bank slow twitch myosin heavy chain myhc type i
    Slow Twitch Myosin Heavy Chain Myhc Type I, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 97/100, based on 421 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/slow twitch myosin heavy chain myhc type i/product/Developmental Studies Hybridoma Bank
    Average 97 stars, based on 421 article reviews
    slow twitch myosin heavy chain myhc type i - by Bioz Stars, 2026-02
    97/100 stars

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    Fiber type-specific analysis of remaining muscle following injury. A Four regions of interests are noted on representative H&E-stained images of mid region of gastrocnemius muscle (see Fig. , images are replicated); scale bar is equal to 1,000 μm. B Representative images of the defect (or analogous location) stained for perilipin 5 (red), <t>MyHC</t> slow (type I myofibers; green), MyHC 2A (type IIa myofibers; orange), and laminin (blue); scale bar is equal to 125 μm. C Percentage of perilipin 5 in defect and border regions of interest (main effect of injury p ≤ 0.046). D In a fiber-type specific approach, the area of perilipin 5 was greatest in type IIb/IIx myofibers (main effect of injury p = 0.005). The fiber type specific E cross-sectional area ( p ≥ 0.064) and F distribution ( p ≥ 0.141). Open circle female, closed male; dots represent an individual animal. Data for percent area of perilipin 5, cross-sectional area, and fiber type distribution were analyzed by two-way ANOVA; statistically significant Main Effects are noted; all p-values are shown within each graph and in Supplemental Table 1
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    Fiber type-specific analysis of remaining muscle following injury. A Four regions of interests are noted on representative H&E-stained images of mid region of gastrocnemius muscle (see Fig. , images are replicated); scale bar is equal to 1,000 μm. B Representative images of the defect (or analogous location) stained for perilipin 5 (red), <t>MyHC</t> slow (type I myofibers; green), MyHC 2A (type IIa myofibers; orange), and laminin (blue); scale bar is equal to 125 μm. C Percentage of perilipin 5 in defect and border regions of interest (main effect of injury p ≤ 0.046). D In a fiber-type specific approach, the area of perilipin 5 was greatest in type IIb/IIx myofibers (main effect of injury p = 0.005). The fiber type specific E cross-sectional area ( p ≥ 0.064) and F distribution ( p ≥ 0.141). Open circle female, closed male; dots represent an individual animal. Data for percent area of perilipin 5, cross-sectional area, and fiber type distribution were analyzed by two-way ANOVA; statistically significant Main Effects are noted; all p-values are shown within each graph and in Supplemental Table 1
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    Fiber type-specific analysis of remaining muscle following injury. A Four regions of interests are noted on representative H&E-stained images of mid region of gastrocnemius muscle (see Fig. , images are replicated); scale bar is equal to 1,000 μm. B Representative images of the defect (or analogous location) stained for perilipin 5 (red), <t>MyHC</t> slow (type I myofibers; green), MyHC 2A (type IIa myofibers; orange), and laminin (blue); scale bar is equal to 125 μm. C Percentage of perilipin 5 in defect and border regions of interest (main effect of injury p ≤ 0.046). D In a fiber-type specific approach, the area of perilipin 5 was greatest in type IIb/IIx myofibers (main effect of injury p = 0.005). The fiber type specific E cross-sectional area ( p ≥ 0.064) and F distribution ( p ≥ 0.141). Open circle female, closed male; dots represent an individual animal. Data for percent area of perilipin 5, cross-sectional area, and fiber type distribution were analyzed by two-way ANOVA; statistically significant Main Effects are noted; all p-values are shown within each graph and in Supplemental Table 1
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    Fiber type-specific analysis of remaining muscle following injury. A Four regions of interests are noted on representative H&E-stained images of mid region of gastrocnemius muscle (see Fig. , images are replicated); scale bar is equal to 1,000 μm. B Representative images of the defect (or analogous location) stained for perilipin 5 (red), <t>MyHC</t> slow (type I myofibers; green), MyHC 2A (type IIa myofibers; orange), and laminin (blue); scale bar is equal to 125 μm. C Percentage of perilipin 5 in defect and border regions of interest (main effect of injury p ≤ 0.046). D In a fiber-type specific approach, the area of perilipin 5 was greatest in type IIb/IIx myofibers (main effect of injury p = 0.005). The fiber type specific E cross-sectional area ( p ≥ 0.064) and F distribution ( p ≥ 0.141). Open circle female, closed male; dots represent an individual animal. Data for percent area of perilipin 5, cross-sectional area, and fiber type distribution were analyzed by two-way ANOVA; statistically significant Main Effects are noted; all p-values are shown within each graph and in Supplemental Table 1
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    Fiber type-specific analysis of remaining muscle following injury. A Four regions of interests are noted on representative H&E-stained images of mid region of gastrocnemius muscle (see Fig. , images are replicated); scale bar is equal to 1,000 μm. B Representative images of the defect (or analogous location) stained for perilipin 5 (red), MyHC slow (type I myofibers; green), MyHC 2A (type IIa myofibers; orange), and laminin (blue); scale bar is equal to 125 μm. C Percentage of perilipin 5 in defect and border regions of interest (main effect of injury p ≤ 0.046). D In a fiber-type specific approach, the area of perilipin 5 was greatest in type IIb/IIx myofibers (main effect of injury p = 0.005). The fiber type specific E cross-sectional area ( p ≥ 0.064) and F distribution ( p ≥ 0.141). Open circle female, closed male; dots represent an individual animal. Data for percent area of perilipin 5, cross-sectional area, and fiber type distribution were analyzed by two-way ANOVA; statistically significant Main Effects are noted; all p-values are shown within each graph and in Supplemental Table 1

    Journal: BMC Musculoskeletal Disorders

    Article Title: Accumulation of lipids after acute direct and indirect traumatic injuries in male and female mice

    doi: 10.1186/s12891-025-09207-5

    Figure Lengend Snippet: Fiber type-specific analysis of remaining muscle following injury. A Four regions of interests are noted on representative H&E-stained images of mid region of gastrocnemius muscle (see Fig. , images are replicated); scale bar is equal to 1,000 μm. B Representative images of the defect (or analogous location) stained for perilipin 5 (red), MyHC slow (type I myofibers; green), MyHC 2A (type IIa myofibers; orange), and laminin (blue); scale bar is equal to 125 μm. C Percentage of perilipin 5 in defect and border regions of interest (main effect of injury p ≤ 0.046). D In a fiber-type specific approach, the area of perilipin 5 was greatest in type IIb/IIx myofibers (main effect of injury p = 0.005). The fiber type specific E cross-sectional area ( p ≥ 0.064) and F distribution ( p ≥ 0.141). Open circle female, closed male; dots represent an individual animal. Data for percent area of perilipin 5, cross-sectional area, and fiber type distribution were analyzed by two-way ANOVA; statistically significant Main Effects are noted; all p-values are shown within each graph and in Supplemental Table 1

    Article Snippet: Within the middle muscle section only, muscles were stained for myosin heavy chain (MyHC) isoform expression MyHC slow (DSHD Cat# BA-D5, RRID: AB_2235587; 5 μg/ml) and MyHC 2A (DSHB Cat# SC-71; RRID: AB_2147165; 5 μg/ml) in conjunction with perilipin 5 (Proteintech Cat#26951-1-AP; RRID: AB_2880699; 5 μg/ml) to analyze changes in fiber type, perilipin 5 expression and co-localization.

    Techniques: Staining

    Fiber type-specific analysis of remaining muscle following injury. A Four regions of interests are noted on representative H&E-stained images of mid region of gastrocnemius muscle (see Fig. , images are replicated); scale bar is equal to 1,000 μm. B Representative images of the defect (or analogous location) stained for perilipin 5 (red), MyHC slow (type I myofibers; green), MyHC 2A (type IIa myofibers; orange), and laminin (blue); scale bar is equal to 125 μm. C Percentage of perilipin 5 in defect and border regions of interest (main effect of injury p ≤ 0.046). D In a fiber-type specific approach, the area of perilipin 5 was greatest in type IIb/IIx myofibers (main effect of injury p = 0.005). The fiber type specific E cross-sectional area ( p ≥ 0.064) and F distribution ( p ≥ 0.141). Open circle female, closed male; dots represent an individual animal. Data for percent area of perilipin 5, cross-sectional area, and fiber type distribution were analyzed by two-way ANOVA; statistically significant Main Effects are noted; all p-values are shown within each graph and in Supplemental Table 1

    Journal: BMC Musculoskeletal Disorders

    Article Title: Accumulation of lipids after acute direct and indirect traumatic injuries in male and female mice

    doi: 10.1186/s12891-025-09207-5

    Figure Lengend Snippet: Fiber type-specific analysis of remaining muscle following injury. A Four regions of interests are noted on representative H&E-stained images of mid region of gastrocnemius muscle (see Fig. , images are replicated); scale bar is equal to 1,000 μm. B Representative images of the defect (or analogous location) stained for perilipin 5 (red), MyHC slow (type I myofibers; green), MyHC 2A (type IIa myofibers; orange), and laminin (blue); scale bar is equal to 125 μm. C Percentage of perilipin 5 in defect and border regions of interest (main effect of injury p ≤ 0.046). D In a fiber-type specific approach, the area of perilipin 5 was greatest in type IIb/IIx myofibers (main effect of injury p = 0.005). The fiber type specific E cross-sectional area ( p ≥ 0.064) and F distribution ( p ≥ 0.141). Open circle female, closed male; dots represent an individual animal. Data for percent area of perilipin 5, cross-sectional area, and fiber type distribution were analyzed by two-way ANOVA; statistically significant Main Effects are noted; all p-values are shown within each graph and in Supplemental Table 1

    Article Snippet: Within the middle muscle section only, muscles were stained for myosin heavy chain (MyHC) isoform expression MyHC slow (DSHD Cat# BA-D5, RRID: AB_2235587; 5 μg/ml) and MyHC 2A (DSHB Cat# SC-71; RRID: AB_2147165; 5 μg/ml) in conjunction with perilipin 5 (Proteintech Cat#26951-1-AP; RRID: AB_2880699; 5 μg/ml) to analyze changes in fiber type, perilipin 5 expression and co-localization.

    Techniques: Staining